2D/3D Profiler | AI Defect Metrology

MICRO INSPEC

2D 3D Profiler

AI Defect Metrology

Image surface detail in 3D at high Z-axis resolution

The BPT Clarity Pro 2D/3D Profiler is a breakthrough optical microscope that reveals surface detail in 3D with unmatched Z-axis resolution—while still performing all conventional optical imaging modes including:

Bright Field
Dark Field
POL
DIC
NIR

One microscope with all the routine imaging modes BF, DF, DIC, POL, OBLIQUE, NIR & FL Plus a Smart-Scope with the next 3 steps in microscopy

Nanometer scale 3D surface imaging and measurements
Artificial Intelligence to perform defect analytics
Multi-microscope unification and data mining to build a useful library of information.

One microscope with all the routine imaging modes BF, DF, DIC, POL, OBLIQUE, NIR & FL Plus a Smart-Scope with the next 3 steps in microscopy

Nanometer scale 3D surface imaging and measurements
Artificial Intelligence to perform defect analytics
Multi-microscope unification and data mining to build a useful library of information.

100X objective
3µ microns line width
Height <1µ micron

Three steps beyond your average optical microscope

The BPT Clarity Pro 2D/3D Profiler is the next generation in complete and smart imaging microscopes. Capable of imaging in Bright Field, Dark Field, DIC, Oblique (directions: 360,180,90 60, 30 degrees) Fluorescence and NIR, it also extends surface roughness characterization down to the angstrom level faster and easier than an AFM.

The BPT Clarity Pro 2D/3D Profiler is the next generation in complete and smart imaging microscopes.

Unique 3D surface roughness image with single digit angstrom resolution equal to an AFM
Artificial Intelligence for defect identification and detection
Multi-system Unification for data mining

Open architecture frame for very large to very small samples
Configured for manual or fully automated operation
Easily accepts most sample loaders including but not limited to wafers loaders

Applications Gallery

Solder Bumps

Step Height

Nano Meter level details

Mesh Overlay on 3D Models

Line Profile

AFM standard with defect below the 50nm step, measured at 9nm

The Future of Life Science Cell Surface Imaging

3D global curvature and local height variation

Solder Bumps

LCD display from and Apple iPad, liquid crystals, transistors and touch screen sensors

RGB Color Filter from an Apple LCD Display

Apple iPad LCD display at 100x objective magnification

Features

Outperforms other systems in the market

Z resolution better than 2 nm

Spatial resolution camera and optics dependent, 50x objective less than half a micron.

Works with consumer cameras and optics

Insensitive to DOF variation

Lowest cost

Research Papers

ASSESSING ADVANTAGES AND DRAWBACKS OF RAPIDLY GENERATED ULTRA-LARGE 3D BREAST CANCER SPHEROIDS: STUDIES WITH CHEMOTHERAPEUTICS

by Austin R. Holub t, Anderson Huo t, Kavil Patel t, Vishal Thakore t, Pranav Chibber and Folarin Erogbogbo *

Pranav Chhibber works as an intern researcher at BrighTex Bio-Photonics.

Graphical abstract

Schematic of ultra-large scaffold showing top view and side view representations

Schematic representation of experimental process.

Breast cancer cell line aggregate test spheroids ( A) 24 h post-cell seeding and (B) 96 h post-DOX treatment (5 μM), taken at 5x objective.

Small spheroid (A) Brightfield images of chemotherapeutic response, (B) percent change in area, and ( C) percent viability.

(A) EVOS Brightfield microscopic images (“raw”) and binary processed images (“processed”) using ImageJ of ultra-large (2000 μm) MCF-7 and MDA-MB-231 spheroids cultured in Prellis Biologics scaffolds pre- (Day 0) and post- (Day 3) treatment with DOX, Dox-NP^Ⓡ, and Doxoves^TM drug formulations (50 µM). Images taken at 4x objective. (Scale bar 2000 µm). Below are graphical presentations of (B) MCF-7 and (C) MDA-MB-231 binary processed images shown in (A).

ATP luminescence readings of (A) MCF-7 and (B) MDA-MB-231 seeded scaffolds exposed molecular DOX, Doxoves^TM, and Dox-NP^Ⓡ drug formulations at concentrations 0.05 µM, 0.5 µM, 5 µM, and 50 µM versus a control.

Live MDA-MB-231 seeded basket imaged with a Zeiss AXIO Observer 5X objective. Cellular fluorescence signal (blue) from Hoechst stain highlights cellular integration to the basket structure.

Computational analysis of MDA-MB-231 seeded scaffold surface. (A) Seeded basket true color dark field image at 5× objective (scale bar: 500 µm), (B) seeded basket colorized image for holistic/global height variation at 5× objective (scale bar: 500 µm), (C) seeded basket colorized image for localized height variation at 85% digital zoom and 5× objective (scale bar: 150 µm), (D ) seeded basket colorized image for holistic height variation at 85% digital zoom and 5× objective (scale bar: 150 µm).

Image surface detail in 3D at high Z-axis resolution

One microscope with all the routine imaging modes BF, DF, DIC, POL, OBLIQUE, NIR & FL Plus a Smart-Scope with the next 3 steps in microscopy

One microscope with all the routine imaging modes BF, DF, DIC, POL, OBLIQUE, NIR & FL Plus a Smart-Scope with the next 3 steps in microscopy

Three steps beyond your average optical microscope

Applications Gallery

Features

Research Papers

ASSESSING ADVANTAGES AND DRAWBACKS OF RAPIDLY GENERATED ULTRA-LARGE 3D BREAST CANCER SPHEROIDS: STUDIES WITH CHEMOTHERAPEUTICS

by Austin R. Holub t, Anderson Huo t, Kavil Patel t, Vishal Thakore t, Pranav Chibber and Folarin Erogbogbo *

Pranav Chhibber works as an intern researcher at BrighTex Bio-Photonics.

Brochure

BPT 2D 3D Profiler System Brochure